17-102-46MI
MilliporeSigma™ Upstate™ Histone H2A.Bbd, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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Antigen
Histone H2A.Bbd
Classification
Polyclonal
Formulation
Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL . Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide.ChIP primers, PGK1. One vial containing 75μL of 5μM of each primer specific for human PGK1. FOR: CCT TCC CCC ACG CTC CTA; REV: TTG TGC AGG AAC CTG TGA GAT G
Gene Symbols
H2A.Bbd, H2AFB1
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_001017990
Includes
This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Accession No.
P0C5Y9
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody recognizes human Histone H2A.Bbd.
Target Species
Human, Mouse
Related Products
Description
- Specifically detects Histone H2A.Bbd Clone: in Human, Mouse samples, and it is validated for ChIP, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blotting Histone H2A.Bbd (Barr body-deficient), also known as histone H2A-Bbd type 1, is an atypical histone variant
- This histone variant associates with H2B and replaces histone H2A in the nucleosome
- In contrast to other H2A histones, this variant lacks conserved residues that are targets of post-translational modifications
- Consequently this H2A.Bbd variant is about 15 amino acids shorter and only 48% similar to the canonical H2A
- The major differences between the two are found in the docking domain that links H2A -H2B to the H3-H4 tetramer
- Nucleosomes containing this histone adopt a more relaxed structure and protect ∽118 base pairs of DNA instead of the 147 base pairs found in classical nucleosomes
- This variant histone is associated with active X chromosomes and autosomes, and is typically absent from inactive X chromosomes.
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Antigen: Histone H2A.Bbd
Classification: Polyclonal
Formulation: Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL . Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide.ChIP primers, PGK1. One vial containing 75μL of 5μM of each primer specific for human PGK1. FOR: CCT TCC CCC ACG CTC CTA; REV: TTG TGC AGG AAC CTG TGA GAT G
Gene Symbols: H2A.Bbd, H2AFB1
Immunogen: KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_001017990
Includes: This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P0C5Y9
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody recognizes human Histone H2A.Bbd.
Target Species: Human, Mouse
Antigen:
Histone H2A.Bbd
Classification:
Polyclonal
Formulation:
Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL . Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide.ChIP primers, PGK1. One vial containing 75μL of 5μM of each primer specific for human PGK1. FOR: CCT TCC CCC ACG CTC CTA; REV: TTG TGC AGG AAC CTG TGA GAT G
Gene Symbols:
H2A.Bbd, H2AFB1
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_001017990
Includes:
This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P0C5Y9
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody recognizes human Histone H2A.Bbd.
Target Species:
Human, Mouse
Antigen: LSF
Classification: Polyclonal
Formulation: Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
Gene Symbols: TFCP2, LSF, SEF
Immunogen: Linear peptide corresponding to amino acids 310-335 of human LSF.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_005644
Includes: This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q12800
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Target Species: Human, Mouse
Antigen:
LSF
Classification:
Polyclonal
Formulation:
Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
Gene Symbols:
TFCP2, LSF, SEF
Immunogen:
Linear peptide corresponding to amino acids 310-335 of human LSF.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_005644
Includes:
This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q12800
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Target Species:
Human, Mouse
Antigen: SOX-2α
Classification: Monoclonal
Formulation: Anti-SOX-2 (mouse monoclonal). One vial containing 75μg of protein G purified monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Mouse IgG. One vial containing 125μg purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols: SOX2
Immunogen: Recombinant protein corresponding to human SOX-2.
Quantity: 25 Assays
Research Discipline: Stem Cell Research
Gene ID (Entrez): NP_003097
Includes: This ChIPAb+ SOX-2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P48431
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Target Species: Human, Mouse
Antigen:
SOX-2α
Classification:
Monoclonal
Formulation:
Anti-SOX-2 (mouse monoclonal). One vial containing 75μg of protein G purified monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Mouse IgG. One vial containing 125μg purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols:
SOX2
Immunogen:
Recombinant protein corresponding to human SOX-2.
Quantity:
25 Assays
Research Discipline:
Stem Cell Research
Gene ID (Entrez):
NP_003097
Includes:
This ChIPAb+ SOX-2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P48431
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Target Species:
Human, Mouse
Antigen: FOXA2α
Classification: Monoclonal
Formulation: Anti-FOXA2 (mouse monoclonal),. One vial containing 100μL of purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.175mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
Gene Symbols: FOXA2, HNF3B, TCF3B
Immunogen: Recombinant protein corresponding to the human FOXA2.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_068556
Includes: This ChIPAb+ FOXA2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9Y261
Host Species: Mouse
Purification Method: Protein G
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Target Species: Human, Mouse
Antigen:
FOXA2α
Classification:
Monoclonal
Formulation:
Anti-FOXA2 (mouse monoclonal),. One vial containing 100μL of purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.175mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
Gene Symbols:
FOXA2, HNF3B, TCF3B
Immunogen:
Recombinant protein corresponding to the human FOXA2.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_068556
Includes:
This ChIPAb+ FOXA2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9Y261
Host Species:
Mouse
Purification Method:
Protein G
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Target Species:
Human, Mouse