17-102-52MI
MilliporeSigma™ Upstate™ LSF, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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Antigen
LSF
Classification
Polyclonal
Formulation
Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
Gene Symbols
TFCP2, LSF, SEF
Immunogen
Linear peptide corresponding to amino acids 310-335 of human LSF.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_005644
Target Species
Human, Mouse
Applications
ChIP Assay, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q12800
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Includes
This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Related Products
Description
- Specifically detects LSF Clone: in Human, Mouse samples, and it is validated for ChIP, Western Blotting Transcription factor LSF (late simian virus 40 factor) also known as transcription factor LSF, CP2, LBP-1c, and SEF is expressed in a variety of cell types including brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung
- LSF is essential for cell cycle progression at the G1/S transition after reentry into the cell cycle from G0
- LSF plays an important role in DNA synthesis and cell survival
- A major cellular target of LSF is the thymidylate synthase (TS) gene coding for the rate limiting enzyme in the production of dTTP
- Disruption of LSF function results in apoptosis at S phase or to cell cycle arrest at the G1/S transition.
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Antigen: LSF
Classification: Polyclonal
Formulation: Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
Gene Symbols: TFCP2, LSF, SEF
Immunogen: Linear peptide corresponding to amino acids 310-335 of human LSF.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_005644
Target Species: Human, Mouse
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q12800
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Includes: This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen:
LSF
Classification:
Polyclonal
Formulation:
Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
Gene Symbols:
TFCP2, LSF, SEF
Immunogen:
Linear peptide corresponding to amino acids 310-335 of human LSF.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_005644
Target Species:
Human, Mouse
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q12800
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Includes:
This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: SOX-2α
Classification: Monoclonal
Formulation: Anti-SOX-2 (mouse monoclonal). One vial containing 75μg of protein G purified monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Mouse IgG. One vial containing 125μg purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols: SOX2
Immunogen: Recombinant protein corresponding to human SOX-2.
Quantity: 25 Assays
Research Discipline: Stem Cell Research
Gene ID (Entrez): NP_003097
Target Species: Human, Mouse
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Gene Accession No.: P48431
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Includes: This ChIPAb+ SOX-2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen:
SOX-2α
Classification:
Monoclonal
Formulation:
Anti-SOX-2 (mouse monoclonal). One vial containing 75μg of protein G purified monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Mouse IgG. One vial containing 125μg purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols:
SOX2
Immunogen:
Recombinant protein corresponding to human SOX-2.
Quantity:
25 Assays
Research Discipline:
Stem Cell Research
Gene ID (Entrez):
NP_003097
Target Species:
Human, Mouse
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
P48431
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Includes:
This ChIPAb+ SOX-2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: FOXA2α
Classification: Monoclonal
Formulation: Anti-FOXA2 (mouse monoclonal),. One vial containing 100μL of purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.175mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
Gene Symbols: FOXA2, HNF3B, TCF3B
Immunogen: Recombinant protein corresponding to the human FOXA2.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_068556
Target Species: Human, Mouse
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q9Y261
Host Species: Mouse
Purification Method: Protein G
Regulatory Status: RUO
Primary or Secondary: Primary
Includes: This ChIPAb+ FOXA2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen:
FOXA2α
Classification:
Monoclonal
Formulation:
Anti-FOXA2 (mouse monoclonal),. One vial containing 100μL of purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.175mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
Gene Symbols:
FOXA2, HNF3B, TCF3B
Immunogen:
Recombinant protein corresponding to the human FOXA2.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_068556
Target Species:
Human, Mouse
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q9Y261
Host Species:
Mouse
Purification Method:
Protein G
Regulatory Status:
RUO
Primary or Secondary:
Primary
Includes:
This ChIPAb+ FOXA2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen: N-CoR
Classification: Polyclonal
Formulation: Anti-N-CoR (Rabbit Polyclonal). One vial containing 75μg of affinity purified polyclonal in buffer containing 0.1M Tris-glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL. Normal Rabbit IgG One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, p21. One vial containing 75μL of each primer (5μM) specific for the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: CCC ACA GCA GAG GAG AAA GAA; REV: CTG GAA ATC TCT GCC CAG ACA
Gene Symbols: Ncor1, Rxrip13
Immunogen: KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_035438
Target Species: Human, Mouse
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q60974.
Host Species: Rabbit
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Includes: This ChIPAb+ N-CoR -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Antigen:
N-CoR
Classification:
Polyclonal
Formulation:
Anti-N-CoR (Rabbit Polyclonal). One vial containing 75μg of affinity purified polyclonal in buffer containing 0.1M Tris-glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL. Normal Rabbit IgG One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, p21. One vial containing 75μL of each primer (5μM) specific for the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: CCC ACA GCA GAG GAG AAA GAA; REV: CTG GAA ATC TCT GCC CAG ACA
Gene Symbols:
Ncor1, Rxrip13
Immunogen:
KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_035438
Target Species:
Human, Mouse
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q60974.
Host Species:
Rabbit
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Includes:
This ChIPAb+ N-CoR -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles