17-656-MI
MilliporeSigma™ Upstate™ Sox-2, clone 6F1.2α, 6F1.2, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-656-MI-Each-of-1 | In Stock | ₹ 52,449.99 |
17-656-MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,449.99
GST (18%): ₹ 9,440.998
Total Price: ₹ 61,890.988
Antigen
Sox-2, clone 6F1.2α
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
P48431
Host Species
Mouse
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_003097
Includes
This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype
IgG2b κ
Applications
ChIP Assay, Dot Blot, Immunocytochemistry, Western Blot
Clone
6F1.2
Formulation
Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG). One vial containing 100μg of protein A purified antibody in 140μL of 0.02M phosphate buffer pH 7.6 with 0.25M NaCl containing 0.1% sodium azide, and 30% glycerol. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols
SOX2; ANOP3; MGC2413; MCOPS3
Immunogen
Recombinant GST Fusion protein.
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes Sox-2, MW: ∽34kDa.
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Specifically detects Sox-2, clone 6F1.2α Clone: 6F1.2 in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, Western Blotting All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time
- Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context
- The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context
- Each set also includes a negative control antibody to ensure specificity of the ChIP reaction
- The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95bp region within the promoter of the human nanog gene
- The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable 'per ChIP' reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin
- Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential
- It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
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Antigen: Sox-2, clone 6F1.2α
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: P48431
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003097
Includes: This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2b κ
Applications: ChIP Assay, Dot Blot, Immunocytochemistry, Western Blot
Clone: 6F1.2
Formulation: Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG). One vial containing 100μg of protein A purified antibody in 140μL of 0.02M phosphate buffer pH 7.6 with 0.25M NaCl containing 0.1% sodium azide, and 30% glycerol. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols: SOX2; ANOP3; MGC2413; MCOPS3
Immunogen: Recombinant GST Fusion protein.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes Sox-2, MW: ∽34kDa.
Target Species: Human, Mouse
Form: Purified
Antigen:
Sox-2, clone 6F1.2α
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
P48431
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003097
Includes:
This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2b κ
Applications:
ChIP Assay, Dot Blot, Immunocytochemistry, Western Blot
Clone:
6F1.2
Formulation:
Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG). One vial containing 100μg of protein A purified antibody in 140μL of 0.02M phosphate buffer pH 7.6 with 0.25M NaCl containing 0.1% sodium azide, and 30% glycerol. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
Gene Symbols:
SOX2; ANOP3; MGC2413; MCOPS3
Immunogen:
Recombinant GST Fusion protein.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes Sox-2, MW: ∽34kDa.
Target Species:
Human, Mouse
Form:
Purified
Antigen: SUZ12α
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: Q15022
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_056170
Includes: This ChIPAb+ SUZ12 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG1 κ
Applications: ChIP Assay, Dot Blot, Western Blot
Clone: __
Formulation: Anti-SUZ12 (mouse monoclonal IgG). One vial containing 50μg of protein A purified antibody in PBS containing 0.05% sodium azide. May contain 30% glycerol (see certificate of analysis). Normal Mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols: SUZ12; JJAZ1; ChET9
Immunogen: SUZ12, purified antibody is made against human SUZ12.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes SUZ12, Mr 95kDa.
Target Species: Human, Mouse, Rat, Vertebrates
Form: Purified
Antigen:
SUZ12α
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
Q15022
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_056170
Includes:
This ChIPAb+ SUZ12 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG1 κ
Applications:
ChIP Assay, Dot Blot, Western Blot
Clone:
__
Formulation:
Anti-SUZ12 (mouse monoclonal IgG). One vial containing 50μg of protein A purified antibody in PBS containing 0.05% sodium azide. May contain 30% glycerol (see certificate of analysis). Normal Mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols:
SUZ12; JJAZ1; ChET9
Immunogen:
SUZ12, purified antibody is made against human SUZ12.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes SUZ12, Mr 95kDa.
Target Species:
Human, Mouse, Rat, Vertebrates
Form:
Purified
Antigen: EZH2, clone AC22α
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: Q15910
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function, Apoptosis and Cancer
Gene ID (Entrez): NP_004447.2
Includes: EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2a
Applications: ChIP Assay, Western Blot
Clone: __
Formulation: EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
Gene Symbols: EZH2; KMT6
Immunogen: EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes EZH2, MW: ∽85-100kDa.
Target Species: Human, Mouse, Rat, Vertebrates
Form: Purified
Antigen:
EZH2, clone AC22α
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
Q15910
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function, Apoptosis and Cancer
Gene ID (Entrez):
NP_004447.2
Includes:
EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2a
Applications:
ChIP Assay, Western Blot
Clone:
__
Formulation:
EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
Gene Symbols:
EZH2; KMT6
Immunogen:
EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes EZH2, MW: ∽85-100kDa.
Target Species:
Human, Mouse, Rat, Vertebrates
Form:
Purified
Antigen: EEDα
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: O75530
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003788
Includes: This ChIPAb+ EED -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2a κ
Applications: ChIP Assay, Western Blot
Clone: __
Formulation: Anti-EED (Mouse monoclonal IgG). One vial containing 50μg of protein G purified antibody in 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl, containing 0.05% azide. May contain 30% glycerol (see certificate of analysis for details). Normal mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols: EED; WAIT-1
Immunogen: Recombinant fusion protein of full length murine EED tagged with MBP.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes EED, MW: ∽50kDa.
Target Species: Human, Mouse
Form: Purified
Antigen:
EEDα
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
O75530
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003788
Includes:
This ChIPAb+ EED -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2a κ
Applications:
ChIP Assay, Western Blot
Clone:
__
Formulation:
Anti-EED (Mouse monoclonal IgG). One vial containing 50μg of protein G purified antibody in 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl, containing 0.05% azide. May contain 30% glycerol (see certificate of analysis for details). Normal mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols:
EED; WAIT-1
Immunogen:
Recombinant fusion protein of full length murine EED tagged with MBP.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes EED, MW: ∽50kDa.
Target Species:
Human, Mouse
Form:
Purified