17-662-MI
MilliporeSigma™ Upstate™ EZH2, clone AC22α, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-662-MI-Each-of-1 | In Stock | ₹ 53,561.42 |
17-662-MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,561.42
GST (18%): ₹ 9,641.056
Total Price: ₹ 63,202.476
Antigen
EZH2, clone AC22α
Classification
Monoclonal
Formulation
EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
Gene Symbols
EZH2; KMT6
Immunogen
EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes EZH2, MW: ∽85-100kDa.
Target Species
Human, Mouse, Rat, Vertebrates
Form
Purified
Applications
ChIP Assay, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q15910
Host Species
Mouse
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function, Apoptosis and Cancer
Gene ID (Entrez)
NP_004447.2
Includes
EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
Content And Storage
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype
IgG2a
Related Products
Description
- Specifically detects EZH2, clone AC22α Clone: in Human, Mouse, Rat, Vertebrate samples, and it is validated for ChIP, Western Blotting EZH2 is a polycomb group (PcG) protein that is a catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene
- Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively
- Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation
- The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems
- Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes
- EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA
- Regulates the circadian clock via histone methylation at the promoter of the circadian genes
- Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Compare Similar Items
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Antigen: EZH2, clone AC22α
Classification: Monoclonal
Formulation: EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
Gene Symbols: EZH2; KMT6
Immunogen: EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes EZH2, MW: ∽85-100kDa.
Target Species: Human, Mouse, Rat, Vertebrates
Form: Purified
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: Q15910
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function, Apoptosis and Cancer
Gene ID (Entrez): NP_004447.2
Includes: EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2a
Antigen:
EZH2, clone AC22α
Classification:
Monoclonal
Formulation:
EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
Gene Symbols:
EZH2; KMT6
Immunogen:
EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes EZH2, MW: ∽85-100kDa.
Target Species:
Human, Mouse, Rat, Vertebrates
Form:
Purified
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
Q15910
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function, Apoptosis and Cancer
Gene ID (Entrez):
NP_004447.2
Includes:
EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2a
Antigen: EEDα
Classification: Monoclonal
Formulation: Anti-EED (Mouse monoclonal IgG). One vial containing 50μg of protein G purified antibody in 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl, containing 0.05% azide. May contain 30% glycerol (see certificate of analysis for details). Normal mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols: EED; WAIT-1
Immunogen: Recombinant fusion protein of full length murine EED tagged with MBP.
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes EED, MW: ∽50kDa.
Target Species: Human, Mouse
Form: Purified
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Gene Accession No.: O75530
Host Species: Mouse
Quantity: 25 Assays
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): NP_003788
Includes: This ChIPAb+ EED -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype: IgG2a κ
Antigen:
EEDα
Classification:
Monoclonal
Formulation:
Anti-EED (Mouse monoclonal IgG). One vial containing 50μg of protein G purified antibody in 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl, containing 0.05% azide. May contain 30% glycerol (see certificate of analysis for details). Normal mouse IgG. Two vials containing 25μg of purified mouse IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Gene Symbols:
EED; WAIT-1
Immunogen:
Recombinant fusion protein of full length murine EED tagged with MBP.
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes EED, MW: ∽50kDa.
Target Species:
Human, Mouse
Form:
Purified
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Gene Accession No.:
O75530
Host Species:
Mouse
Quantity:
25 Assays
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
NP_003788
Includes:
This ChIPAb+ EED -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype:
IgG2a κ
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