17-100-61MI

MilliporeSigma™ Upstate™ E2F-1α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

Select a Size

Pack Size SKU Availability Price
Each of 1 17-100-61MI-Each-of-1 In Stock ₹ 52,449.99

17-100-61MI - Each of 1

₹ 52,449.99

In Stock

Quantity

1

Base Price: ₹ 52,449.99

GST (18%): ₹ 9,440.998

Total Price: ₹ 61,890.988

Antigen

E2F-1α

Classification

Monoclonal

Formulation

Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT

Gene Symbols

E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3

Immunogen

GST fusion protein corresponding to full-length human E2F-1.

Quantity

25 Assays

Research Discipline

Epigenetics and Nuclear Function

Gene ID (Entrez)

NP_005216

Includes

This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage

-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype

IgG

Applications

ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation

Conjugate

Unconjugated

Gene Accession No.

Q01094

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.

Target Species

Human, Xenopus species

Form

Purified

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Description

  • Specifically detects E2F-1α Clone: in Human, Xenopus samples, and it is validated for ChIP, ChIP-seq, Immunoprecipitation E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter
  • The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity
  • E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

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Show Difference

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MilliporeSigma™

17-100-61MI

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Antigen:
E2F-1α

Classification:
Monoclonal

Formulation:
Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT

Gene Symbols:
E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3

Immunogen:
GST fusion protein corresponding to full-length human E2F-1.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_005216

Includes:
This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Isotype:
IgG

Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation

Conjugate:
Unconjugated

Gene Accession No.:
Q01094

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.

Target Species:
Human, Xenopus species

Form:
Purified

Img

MilliporeSigma™

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Antigen:
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Classification:
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Formulation:
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Gene Symbols:
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Immunogen:
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Quantity:
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Research Discipline:
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Gene ID (Entrez):
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Includes:
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Content And Storage:
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Isotype:
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Gene Accession No.:
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Purification Method:
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Regulatory Status:
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Form:
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MilliporeSigma™

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Antigen:
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Classification:
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Formulation:
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Immunogen:
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Quantity:
96 Assays

Research Discipline:
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Includes:
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Content And Storage:
Store components at the temperatures indicated on the labels

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Micronova™

17-100-812

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Antigen:
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Formulation:
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Immunogen:
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Quantity:
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Research Discipline:
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Gene ID (Entrez):
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Includes:
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Content And Storage:
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Isotype:
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Applications:
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Conjugate:
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Gene Accession No.:
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Host Species:
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Purification Method:
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Regulatory Status:
__

Primary or Secondary:
__

Test Specificity:
__

Target Species:
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Form:
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