Invitrogen™ Dynabeads™ Untouched™ Mouse CD8 Cells Kit

Description

• Dynabeads™ Untouched™ Mouse CD8 Cells supplies both 4.5 μm superparamagnetic Dynabeads (with a polyclonal sheep anti-rat IgG antibody covalently bound to the bead surface) and a monoclonal Antibody Mix, together designed for the depletion of CD8-negative leucocytes from mouse spleen or lymph node single-cell suspensions
• Following the depletion procedure, the negatively isolated, untouched target mouse CD8 + cells are pure, viable, and ready to be used in any downstream application
• • Rapid isolation of untouched mouse CD8 + T cells—no columns required • Pure and viable mouse CD8 + T cells are ready for any downstream application Excellent yield, purity, and viability of recovered cells The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD4 + T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes, and granulocytes
• The Antibody Mix is first added to the sample in a tube to bind the unwanted CD8-negative cells
• The cells are then washed to remove excess antibodies
• Mouse Depletion Dynabeads™ (coated with a rat IgG antibody) are added to bind the antibody-labeled, CD8-negative cells
• After a short incubation, the bead-bound cells are then separated from unbound cells in 1–2 minutes using a magnet The purified target mouse CD8 + cells in the supernatant can be transferred to a new tube for use in any downstream application
• This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD8 + cells
• The protocol can be scaled up according to your sample size.