NOXA Polyclonal Antibody, Invitrogen™

Description

• Reconstitute in 100 μL of sterile water
• Centrifuge to remove any insoluble material
• After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent
• Glycerol (1:1) may be added for an additional stability
• Avoid repetitive freeze/thaw cycles
• Apoptosis is related to many diseases and development
• The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA
• A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins
• Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins
• X-ray irradiation increased p53-dependent Noxa mRNA and protein levels
• Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9
• Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53
• Noxa and PUMA may represent direct mediators of p53-induced apoptosis
• Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.