CD203c Monoclonal Antibody (NP4D6), PE, eBioscience™, Invitrogen™

Description

• Description: This NP4D6 monoclonal antibody reacts with human CD203c, which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3)
• ENPP family members catalyze the hydrolysis of pyrophosphate and phosphodiester bonds to nucleoside 5-monophosphates
• A type II transmembrane protein, CD203c is expressed on basophils and mast cells
• CD203c expression is upregulated on basophils activated by allergen or IgE crosslinking
• Therefore, CD203c is used as an indicator of basophil activation in various IgE-mediated allergic responses
• Applications Reported: This NP4D6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This NP4D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides
• These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins
• Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract
• The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver
• The human mRNA is expressed in glioma cells, prostate, and uterus
• Expression of the human protein has been detected in uterus, basophils, and mast cells
• [provided by RefSeq, Jul 2008]