CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience™, Invitrogen™

Description

• The MAb 439-9B recognizes human CD104, also known as integrin beta 4
• CD104 is a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor
• Multiple splice variants have been identified
• Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions
• The role of CD104 in tumorigenicity is well established
• The MAb 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells
• Moreover, this antibody crossreacts to bovine
• Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting under nonreducing conditions only, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections
• Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors
• Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities
• Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth
• This gene encodes the integrin beta 4 subunit, a receptor for the laminins
• This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma
• Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia
• Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.