CD328 (Siglec7) Monoclonal Antibody (eBioQA79 (QA79)), eBioscience™, Invitrogen™

Description

• Description: The eBioQA79 monoclonal antibody reacts with adhesion inhibitory receptor molecule 1 (AIRM1), also known as sialic acid binding immunoglobulin-like lectin 7 (Siglec-7), p75 and CDw328
• AIRM1 is a cell surface receptor that belongs to the sialoadhesin family and displays homology with the myeloid cell antigen CD33
• p75/AIRM1 expression is confined to NK cells in lymphoid cells
• eBioQA79 crosslinking inhibits NK function
• p75/AIRM1 is expressed at various stages of myeloid differentiation
• Presence of this protein is a useful way to monitor cellular proliferation and determine proliferative states of cells, especially in diseases such as chronic myeloid leukemia
• Applications Reported: This eBioQA79 (QA79) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation
• Applications Tested: This eBioQA79 (QA79) antibody has been tested by flow cytometric analysis
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Putative adhesion molecule that mediates sialic-acid dependent binding to cells
• Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid
• Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide)
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
• In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules
• Mediates inhibition of natural killer cells cytotoxicity
• May play a role in hemopoiesis
• Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).