TOX Monoclonal Antibody (TXRX10), eBioscience™, Invitrogen™

Description

• Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein
• This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells
• TOX expression in thymocytes is upregulated during beta-selection and positive selection
• Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4^+ T cell development as a regulator of the CD4^loCD8^lo to CD4^+CD8^lo transition
• Moreover, TOX has been shown to be involved in the development of NK and LTi cells
• While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection
• Applications Reported: This TXRX10 antibody has been reported for use in immunoprecipitation and immunoblotting (WB)
• Applications Tested: This TXRX10 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse thymocytes at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development
• It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes
• TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent
• TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.