CD281 (TLR1) Monoclonal Antibody (eBioTR23 (TR23)), eBioscience™, Invitrogen™

Description

• Description: The eBioTR23 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281)
• TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function
• Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis
• TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria
• Applications Reported: This eBioTR23 (TR23) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation
• Applications Tested: This eBioTR23 (TR23) antibody has been tested by flow cytometric analysis of mouse TLR1-transfected Baf/3 cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• TLR1 protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity
• TLRs are highly conserved from Drosophila to humans and share structural and functional similarities
• They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity
• The various TLRs exhibit different patterns of expression
• The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain
• The TLRs could constitute an important and unrecognized component of innate immunity in humans
• In mice, TLR1 interacts with TLR2 and co-expression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide
• They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several tri-acylated lipopeptides
• Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for the TLR1 gene.