Bcl-2 Monoclonal Antibody (10C4), eBioscience™, Invitrogen™

Description

• Description: The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2
• Bcl-2 is the founding member of the Bcl-2 family of evolutionarily-conserved proteins that regulate cell death/survival
• Members of the Bcl-2 family include the pro-apoptotic proteins BAD, Bax, and Bim, and the anti-apoptotic proteins Bcl-2, Bcl-xL, and Mcl-1
• Bcl-2 inhibits apoptosis by preventing the release of cytochrome c from the mitochondria
• Mutation of Bcl-2 or overexpression of Bcl-2 due to chromosomal translocation has been identified as a contributing factor in numerous cancers
• In developing thymocytes, Bcl-2 is expressed at high levels in CD4-CD8- double negative and in CD4+ and CD8+ single positive thymocytes; Bcl-2 expression is greatly diminished in CD4+CD8+ double positive thymocytes
• Applications Reported: The 10C4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunoblotting (WB)
• (Fluorochrome conjugated 10C4 is recommended for use in intracellular flow cytometry.)
• Applications Tested: The 10C4 antibody has been tested by western blot and can be used at 1-10 μg/mL
• It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death
• The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis
• Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2
• Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis
• Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells
• Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma
• In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative
• Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends
• The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.