c-Met Monoclonal Antibody (eBioclone 97), eBioscience™, Invitrogen™

Description

• Description: 97 monoclonal antibody was generated against a human c-Met-Ig fusion protein, and reacts with human c-Met (HGFR)-transfected cells
• Human c-Met is a 145 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues
• Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma;
• Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis
• Mutations in human c-Met have been implicated in the development of several maligcies
• Applications Reported: This eBioclone 97 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioclone 97 antibody has been tested by flow cytometric analysis of human c-Met-transfected cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies
• After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3
• There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC
• MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation
• It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling
• MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.