ALDH1L1 Monoclonal Antibody (7G8), eBioscience™, Invitrogen™

Description

• Description: This 7G8 monoclonal antibody reacts with human 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) a protein involved in folate metabolism
• The cytosolic enzyme, with homology to the aldehyde dehydrogenase (ALDH) family, is involved in the conversion of 10-formyltetrahydrofolate, NADP and water to tetrahydrofolate, NADPH and carbon dioxide
• Folate metabolism is essential for cell growth and proliferation and decreased levels of folate have been implicated in neural tube defects in addition to increased cancer risk
• This isoform (1L1) is expressed in glial cells has been identified as a highly specific marker for astrocytes labeling both the cell body and processes
• Applications Reported: This 7G8 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining, and immunocytochemistry
• Applications Tested: This 7G8 antibody has been tested by immunohistochemistry on human FFPE tissue using both high and low pH antigen retrieval
• Both antigen retrieval methods resulted in staining although low pH antigen retrieval is optimal
• The antibody can be used at less than or equal to 1 μg/mL
• It is recommended that anibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide
• The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo
• Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.