Fibronectin Monoclonal Antibody (FN-3), eBioscience™, Invitrogen™

Description

• Description: The FN-3 monoclonal antibody recognizes human fibronectin, a component of the extracellular matrix
• This high molecular weight protein (almost 400 kDa) exists as an extracellular and a soluble plasma form
• Fibronectin functions in cell adhesion and migration via integrins present on the interacting cell
• Fibronectin also associates with collagen, actin and fibrins
• In malignancies, fibronectin protein levels can be altered
• In lung carninomas, expression is decreased
• The FN-3 antibody recognizes a determinant on human cellular but not plasma fibronectin; this recognition is not lost upon trypsin treatment
• Furthermore, FN-3 antibody has been shown to crossreact to bovine fibronectin
• Applications Reported: This FN-3 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections and immunoblotting
• Applications Tested: This FN-3 antibody has been tested by immunohistochemistry on FFPE human placenta (with IHC Antigen Retrieval Solution - Low pH (cat
• 00-4955)) and can be used at less than or equal to 10 μg/mL
• Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa
• In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers
• Fibronectin is most abundant during embryonic development and tissue remodeling
• Fibronectin is also present at high concentrations as a soluble plasma protein
• Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g
• collagen and heparin) and receptors on cells (e.g
• integrins)
• Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix
• Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis
• Fibronectin has been implicated in carcinoma development in lung cancer
• Further, Fibronectin expression is increased especially in non-small cell lung carcinoma
• The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents
• The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants
• However, the full-length nature of some Fibronectin variants has not been determined.