CD47 Monoclonal Antibody (B6H12), Functional Grade, eBioscience™, Invitrogen™

Description

• The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D
• Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72
• Interaction of these two proteins diminishes the negative signaling normally effected by CD72
• CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types
• Antigen expression has been reported in Spleen, thymus and l CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions
• Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins
• Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix
• CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction
• CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes
• CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells
• Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.