CD47 Monoclonal Antibody (2D3), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 2D3 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin
• CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain
• CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61
• Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages
• CD47 is the receptor for thrombospondin
• T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin)
• Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex)
• Expression is also found in non-hematopoietic cells
• Applications Reported: This 2D3 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB) under nonreducing conditions
• Applications Tested: This 2D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions
• Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins
• Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix
• CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction
• CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes
• CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells
• Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.