CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Functional Grade, eBioscience™, Invitrogen™

Description

• The MAb CXCR3-173 recognizes mouse CD183 also known as CXCR3
• CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL9 (mig), CXCL10 (IP-10)and CXCL11 (ITAC)
• CD183 as been shown to play a role in CD4 T cell responses to grafts
• CXCR3 knockout mice have compromised allograft rejection responses
• Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarized cells
• The antibody CXCR3-173 has been shown to affect chemotaxis in response to ligand
• The presence of ligand eliminates staining with the antibody
• In vivo addition of the antibody delays cardiac and pancreatic islet allograft rejection
• This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant)
• IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues
• Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration
• Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein
• Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors
• As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration
• Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface
• This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues
• In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells
• Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases
• Multiple transcript variants encoding different isoforms have been found for this gene.