CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience™, Invitrogen™

Description

• The eBioT21/8 MAb reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES
• Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells
• CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120
• Binding of the eBioT21/8 MAb displays donor variability and may be affected by sub-optimal staining conditions
• Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells
• Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors
• Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells
• CCR5 is required for the infection by HIV-1, HIV-2, and SIV
• The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1
• CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells
• The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites
• CCR5 messenger RNA is expressed in lymphoid organs and monocytes.