CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), APC, eBioscience™, Invitrogen™

Description

• Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1
• CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta
• Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection
• Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells
• The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5
• Applications Reported: This HM-CCR5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HM-CCR5 antibody has been tested by flow cytometric analysis of thioglycolate-induced peritoneal exudate cells
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells
• Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors
• Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells
• CCR5 is required for the infection by HIV-1, HIV-2, and SIV
• The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1
• CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells
• The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites
• CCR5 messenger RNA is expressed in lymphoid organs and monocytes.