CD186 (CXCR6) Monoclonal Antibody (DANID2), APC, eBioscience™, Invitrogen™

Description

• Description: This DANID2 monoclonal antibody recognizes mouse CD186, also known as CXCR6
• This DANID2 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells
• Applications Reported: This DANID2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DANID2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser CD186, also known as CXCR6, is a G protein-coupled chemokine receptor reported to be one of the cellular receptors of HIV and SIV
• CD186 is expressed by NKT cells, various subtypes of T cells with activated and memory phenotypes, and some plasma cells
• It has been reported that mouse liver NK cells, but not naïve or splenic NK cells, express CD186
• The ligand of CD186, CXCL16, is expressed by macrophages, dendritic cells and liver endothelial cells
• Being highly expressed on NKT cells, CD186 is often used as a proxy marker of this cell subset.