CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Ultra Violet™ 395, eBioscience™, Invitrogen™

Description

• This 3DS223H mAb recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3)
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells)
• This 3DS223H antibody will recognize a formaldehyde-fixed epitope
• Applications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5μL (0.5μg) per test BUV395 is a dye that emits at 395 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Excitation: 350 nm; Emission: 395 nm; Laser: Ultraviolet Laser
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).