3855702

PKA [RI] Mouse, Unlabeled, Clone: 18, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

PKA [RI]

Concentration

250μg/mL

Classification

Monoclonal

Host Species

Mouse

Research Discipline

Cell Biology

Formulation

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype

IgG2b

Purification Method

Affinity Purified

Clone

18

Applications

Western Blot

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Canine, Chicken, Frog, Human, Mouse, Rat

Immunogen

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Primary or Secondary

Primary

Content And Storage

Store undiluted at -20°C.

Description

  • cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R)
  • Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ
  • These subunits define type I and II cAMP-dependent protein kinases
  • Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active
  • Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ)
  • Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity
  • Most cells express both type I and type II PKAs
  • Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominant in nervous and adipose tissues
  • The levels of expression of the different subunits vary according to cell and tissue type
  • Bioimaging, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

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3855702

Mouse Monoclonal Antibody...


Antigen:
PKA [RI]

Concentration:
250μg/mL

Classification:
Monoclonal

Host Species:
Mouse

Research Discipline:
Cell Biology

Formulation:
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype:
IgG2b

Purification Method:
Affinity Purified

Clone:
18

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Canine, Chicken, Frog, Human, Mouse, Rat

Immunogen:
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at -20°C.

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Research Discipline:
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Formulation:
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Isotype:
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Purification Method:
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Clone:
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Applications:
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Conjugate:
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Regulatory Status:
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Target Species:
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