PKCι Mouse, Unlabeled, Clone: 23, BD

Description

• The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion
• At least eleven isozymes have been described
• These proteins are products of multiple genes and alternative splicing
• PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions(V)
• The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca 2 + , phospholipid, diacylglycerol, or phorbol ester
• However, the novel PKC (nPKC) subfamily members (δ, ε, η and θ isoforms) and the atypical PKC (aPKC) subfamily members (ζ, ι and γ isoforms) are Ca 2 + independent and lack the C2 domain
• The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters
• They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC
• The C-terminal region of PKC contains the catalytic domain
• The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors
• PKCι was isolated from a human kidney cDNA library
• It is highly expressed in brain and lung, but it is also seen in many other tissues at lower levels
• PKCι shows the most similarity to PKCζ , 72% overall identity
• These two enzymes share a highly conserved pseudosubstrate sequence, the absence of a Ca 2 + -binding region, and the presence of only one zinc finger-like domain
• It is thought that PKC? has a role in the secretory response to nutrients
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting