PKCθ Mouse anti-Human, Unlabeled, Clone: 27, BD

Description

• The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion
• At least eleven isozymes have been described
• These proteins are products of multiple genes and alternative splicing
• PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V)
• The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca 2+ , phospholipid, diacylglycerol, or phorbol ester
• However, the novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) and the atypical PKC (aPKC) subfamily members (ζ, ι, and λ isoforms) are Ca 2+ -independent and lack the C2 domain
• The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters
• They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members
• The C-terminal region of PKC contains the catalytic domain
• The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors
• PKCθ transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes
• PKCθ mRNA is readily detectable in skeletal muscle, lung and brain
• However, PKCθ expression is not detected in several human carcinoma cell lines
• Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells
• Host Species: Mouse Clone: 27 Isotype: IgG2a κ Species Reactivity: Human Immunogen: Human PKCθ aa
• 21-217 Formula Weight [Chemical]: 79kDa Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting