CD27 Mouse anti-Human, Brilliant Violet 605, Clone: L128, BD

Description

• The L128 monoclonal antibody specifically binds to human CD27
• CD27 is a 55-kDa disulfide-linked dimer that is a member of the nerve growth factor (NGF) super family
• This family also includes CD40, rat OX40, tumor necrosis factor (TNF) receptors and CD95 (Fas)
• With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes
• Present on most peripheral blood T lymphocytes and medullary thymocytes, the CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa
• It is also detected on a subpopulation of approximately 33% of circulating B lymphocytes
• Following exposure to antigens, CD45RA+ T lymphocytes respond by upregulating the CD27 antigen
• After maximal stimulation, the CD27 antigen cannot be re-expressed on long-term cultures or on CD45RA-CD27+ T lymphocytes
• The CD4+CD27- population is contained within the memory CD45RO+ subset that proliferates after exposure to allergens
• Two subpopulations of B lymphocytes bearing the CD27 antigen secrete IgM (δ+) and IgG (δ-)
• For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment
• Fluorescent dye interactions may cause staining artifacts which may affect data interpretation
• The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions
• More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat
• No
• 563794).