CD200 Monoclonal Antibody (OX104), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody OX104 recognizes human CD200 also known as OX2
• CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain
• CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells
• The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNFalpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes
• A role has also been suggested in maternal tolerance as expression of CD200 is also present on the trophoblast
• Applications Reported: This OX104 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This OX104 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor 450 is an alternative to Pacific Blue
• eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser CD200 is a type-1 membrane glycoprotein that contains two immunoglobulin domains, and belongs to the immunoglobulin superfamily
• CD200 is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) and is implicated in locally preventing macrophage activation
• Distant, but recognizable, homologues of CD200 have been identified in many herpesviruses and poxviruses
• Studies of the related genes in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues
• Multiple alternatively spliced transcript variants that encode different isoforms of CD200 have been found
• Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.