CD63 Monoclonal Antibody (H5C6), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins
• CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils
• However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes
• This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells
• Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response
• Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types
• CD63 is also present in the plasma membrane, usually following cell activation
• Hence, CD63 has become a widely used basophil activation marker
• In mast cells, however, CD63 exposition does not need their activation
• CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels
• CD63 also serves as a T-cell costimulation molecule
• Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas
• CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation
• CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells
• CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40
• Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.