CD107b (LAMP-2) Monoclonal Antibody (M3/84), PE, eBioscience™, Invitrogen™

Description

• Description: The M3/84 monoclonal antibody reacts with mouse Mac-3, the 110 kDa protein expressed by mononuclear phagocytes
• The Mac-3 antigen is expressed intracellularly by bone marrow monocyte lineage and is upregulated during macrophage differentiation
• Peritoneal and tissue macrophages, dendritic cells, and endothelial cells express this antigen on their surface
• It is reported that Mac-3 may be identical to CD107b
• Applications Reported: The M3/84 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The M3/84 antibody has been tested by flow cytometric analysis of mouse bone marrow and thioglycolate-elicited peritoneal exudates cells and can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins
• LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome
• Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide
• Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa
• LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail
• LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy
• LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail
• The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C
• LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane
• The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation
• LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).