50-112-4143

CD146 Monoclonal Antibody (P1H12), eFluor™ 450, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-4143-Each-of-1 In Stock ₹ 30,373.80

50-112-4143 - Each of 1

₹ 30,373.80

In Stock

Quantity

1

Base Price: ₹ 30,373.80

GST (18%): ₹ 5,467.284

Total Price: ₹ 35,841.084

Antigen

CD146

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P43121, Q8R2Y2

Gene Symbols

MCAM

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

100342848, 4162, 489368, 84004

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

P1H12

Conjugate

eFluor 450

Gene

MCAM

Gene Alias

1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Canine, Human, Mouse, Rabbit

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins
  • The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma)
  • Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146
  • The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative
  • Expression can be found on activated lymphocytes
  • The protein is heavily glycosylated with more than 50% of the mass from carbohydrates
  • The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat
  • Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.125 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • eFluor™ 450 is an alternative to Pacific Blue™
  • CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily
  • CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types
  • In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly
  • CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue
  • CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread
  • Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development
  • CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Compare Similar Items

Show Difference

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Invitrogen

50-112-4143

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Antigen:
CD146

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P43121, Q8R2Y2

Gene Symbols:
MCAM

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
100342848, 4162, 489368, 84004

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
P1H12

Conjugate:
eFluor 450

Gene:
MCAM

Gene Alias:
1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Canine, Human, Mouse, Rabbit

Product Type:
Antibody

Isotype:
IgG1 κ

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Antigen:
CD223 (LAG-3)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P18627

Gene Symbols:
LAG3

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
3902

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
3DS223H

Conjugate:
eFluor 450

Gene:
LAG3

Gene Alias:
Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3lymphocyte activating 3; soluble LAG3

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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Fischer Scientific

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Antigen:
Rat IgG1 kappa

Classification:
__

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
__

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Control, Flow Cytometry

Clone:
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Conjugate:
eFluor 450

Gene:
__

Gene Alias:
Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Isotype Control (Primary)

Target Species:
Not Applicable

Product Type:
__

Isotype:
IgG1 κ

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Antigen:
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Classification:
Monoclonal

Concentration:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q61098

Gene Symbols:
Il18r1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
16182

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
P3TUNYA

Conjugate:
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Gene:
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Gene Alias:
CD218 antigen-like family member A; CD218a; CDw218a; cytokine receptor; IL1 receptor-related protein; Il18r1; IL-18R1; IL-18R-1; IL18RA; Il18ralpha; IL-18R-alpha; IL18Ralpha2; IL1RRP; IL-1Rrp; IL1R-rp; interleukin 1 receptor related protein; interleukin 18 receptor 1; Interleukin-18; interleukin-18 receptor 1; Interleukin-18 receptor alpha

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ