CD146 Monoclonal Antibody (P1H12), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins
• The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma)
• Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146
• The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative
• Expression can be found on activated lymphocytes
• The protein is heavily glycosylated with more than 50% of the mass from carbohydrates
• The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat
• Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily
• CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types
• In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly
• CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue
• CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread
• Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development
• CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.