CD11c Monoclonal Antibody (118/A5), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 118/A5 recognizes human CD11c, a 150 kD type I transmembrane protein which is also known as integrin alpha X
• Integrins are heterodimeric integral membrane proteins made up of an alpha and a beta chain and are involved in cell adhesion
• The CD11/CD18 adhesion molecules consist of three heterodimers sharing a common beta subunit (CD18) with a distinct alpha subunit (CD11a, CD11b, CD11c)
• The CD11c/CD18 complex is expressed on dendritic cells (highly expressed on type 1 myeloid DCs), monocytes, macrophages, granulocytes, NK cells, and subsets of T and B cells
• CD11c is also expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias
• CD11c plays a role in adhesion and CTL killing through its interactions with CD54, iC3b and fibrinogen
• Applications Reported: This 118/A5 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy
• Applications Tested: This 118/A5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• CD11, along with CD18, form a heterodimer adhesion molecule
• In particular, CD11 is composed of CD11a, CD11b and CD11c
• CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils
• CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes
• CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.