CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin
• CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions
• Expression of CD43 is found on most leukocytes except resting B lymphocytes
• Proteolytic processing upon activation decreases surface expression
• CD43 is involved in activation of T cells, B cells, NK cells, and monocytes
• The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages
• Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells
• CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation
• By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic
• CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors
• Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability
• CD43 appears to be an important modulator of leukocyte functions
• Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.