MHC Class II Monoclonal Antibody (HIS19), eBioscience™, Invitrogen™

Description

• Description: The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B
• This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains)
• The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages
• Applications Reported: The HIS19 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections
• Applications Tested: The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells
• Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells
• The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.