CD153 Monoclonal Antibody (RM153), eBioscience™, Invitrogen™

Description

• Description: The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand)
• This 40 kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily
• Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation
• Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation
• Signaling through CD153 is important in costimulation of pre-activated T cells
• Applications Reported: The RM153 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections
• It has also been reported in blocking of ligand binding
• (Please use Functional Grade purified RM153, cat
• 16-1531, in functional assays.)
• Applications Tested: The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• The ligand for CD30 is CD30L (CD153)
• The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death
• CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas
• CD30 acts as a costimulatory molecule in thymic negative selection
• In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation
• In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.