CD154 (CD40 Ligand) Monoclonal Antibody (24-31), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L)
• CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells
• Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction
• CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry
• 24-31 cross-reacts with the cynomolgus monkey CD154
• Applications Reported: 24-31 has been reported for use in flow cytometric analysis
• 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro
• Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of activated normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• Filtration: 0
• CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells
• The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines
• CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes
• It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.