CD267 (TACI) Monoclonal Antibody (11H3), eBioscience™, Invitrogen™

Description

• Description: The 11H3 antibody reacts with human TACI, also known as tumor necrosis factor receptor 13B
• TACI, like BCMA, binds BAFF and April
• TACI and its ligands regulate humoral immune responses, activate NF-kB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases
• TACI mRNA and protein are found predominantly in B-cells and correlate with BLyS binding in a panel of B-cell lines
• TACI interacts with nanomolar affinity with the BLyS-related tumor necrosis factor homologue APRIL for which no clear in vivo role has been described
• 11H3 does not block binding of BAFF/BLyS to TACI
• Applications Reported: This 11H3 antibody has been reported for use in flow cytometric analysis
• (Please use Functional Grade purified 11H3, cat
• 16-9217, in functional assays.)
• Applications Tested: This 11H3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Members in the TNF superfamily regulate immune responses and induce apoptosis
• Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively
• BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion
• April regulates immunological and non-immunological cell growth
• Three receptors, BCMA, TACI, and BAFF-R, for BAFF and April were recently identified
• TACI, like BCMA, binds BAFF and April
• TACI and its ligands regulate humoral immune responses, activate NF-kappa-B and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.