CD268 (BAFF Receptor) Monoclonal Antibody (8A7), eBioscience™, Invitrogen™

Description

• Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells
• The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression
• Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression
• Applications Reported: The 8A7 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining
• It has also been reported for use in functional assays
• (Please use Functional Grade purified 8A7, cat
• 16-9117, in functional assays.)
• Applications Tested: The 8A7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene
• The BAFFR gene is mapped to chromosome 22q13
• 1-q13
• 31
• It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein
• B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population
• BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.