CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily
• CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex
• Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction
• Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation
• The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays
• Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis and functional assays
• Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells
• Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells
• CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development
• Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival
• Soluble forms of CD137 may provide negative control mechanism for some immune responses.