RAE1 delta Monoclonal Antibody (RD-41), eBioscience™, Invitrogen™

Description

• Description: The RD-41 monoclonal antibody reacts with the murine Rae1delta
• Rae-1delta is one of several known murine NKG2D ligands that include RAE-1 molecules (alpha, beta, ε, gamma and delta), H60 and MULT-1
• Expression of NKG2D ligands is low or absent on normal adult tissues
• However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D
• Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands
• The RD-41 antibody has been reported to block tetramer staining
• Applications Reported: This RD-41 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections and ELISA
• Applications Tested: This RD-41 antibody has been tested by flow cytometric analysis of dendritic cell line
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Rae-1 delta is one of several known murine NKG2D ligands that include RAE-1 molecules, H60 and MULT-1
• Expression of NKG2D ligands is low or absent on normal adult tissues
• However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D
• Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands.