CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144)
• VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail
• VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions
• VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures
• Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells
• Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections
• Intravenous injection of BV13 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs
• Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells
• This can be used at less than or equal to 1 μg per test
• VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer
• VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions
• An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.