CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells
• EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins
• EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation
• Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis
• Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods
• The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells
• Applications Reported: This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
• The eBio1560 monoclonal antibody has been reported to block protein C activation by thrombin on cells expressing both thrombomodulin and CD201
• Applications Tested: This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells
• This can be used at less than or equal to 1 μg per test
• The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway
• The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C
• Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.