HLA-G Monoclonal Antibody (87G), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses
• HLA-G has seven reported isoforms
• The antibody 87G recognizes both HLA-G1 and the solube HLA-G5
• Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system
• Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL
• Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs
• The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158
• Recent studies have shown a role for HLA-G in tolerance and maintece of transplanted organs
• Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 87G antibody has been pre-titrated and tested by flow cytometric analysis of stimulated U937 cells
• This can be used at 5 μL (0.125 μg) per test
• HLA-G belongs to the HLA class I heavy chain paralogues
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
• The heavy chain is anchored in the membrane
• HLA-G is expressed on fetal derived placental cells
• The heavy chain is approximately 45 kDa and its gene contains 8 exons
• Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.