TCR V beta 8 Monoclonal Antibody (JR-2), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This JR-2 monoclonal antibody reacts with the human T cell receptor (TCR) Vbeta 8 allele
• Composed of an alpha and beta chain, TCR specificity is typically determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement
• Vβ expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection
• More recently, assessment of TCR Vbeta expression has been used to phenotype T cell clonality in CD3+/TCRalpha beta+ large granular lymphocyte leukemias
• A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells
• Applications Reported: This JR-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This JR-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response
• TCR recognition of self-peptides has been linked to autoimmune disease
• Mutant self-peptides have been associated with tumors
• Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome
• Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)
• The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease
• Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP)
• A small number of T cells lack both CD4 and CD8 (double negative, DN)
• Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders
• Gamma-delta T cells are primarily found within the epithelium
• They show less TCR diversity and recognize antigens differently than alpha-beta T cells
• Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.