CD62L (L-Selectin) Monoclonal Antibody (DREG.200), eBioscience™, Invitrogen™

Description

• Description: Leukocyte-Endothelial Cell Adhesion Molecule-1 belongs to the selectin family of adhesion molecule
• Together with E-selectin, P-selectin and L-selectin mediates the initial interactions of leukocytes with endothelial cells
• Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer
• L-selectin in this aspect mediates rolling of PMN's on endothelial cells
• The potential binding partners of L-selectin carry a negative charge, probably a sialic acid and/or sulphate, and may contain mannose and fucose
• In addition, L-selectin may also interact with E-selectin which is expressed on cytokine-activated endothelial cells
• L-selectin is constitutively expressed on most leukocytes in a seemingly functional form
• It is required for the binding of lymphocytes to the high endothelial venules of peripheral lymph nodes and for the invasion of neutrophils into sites of inflammation
• When neutrophils are activated, L-selectin is shed by proteolytic cleveage near the transmembrane span
• Lymphocytes and monocytes can also shed L-selectin upon activation although the kinetics are significantly lower
• A broad range of activating agents are effective in inducing this response
• The shed form of sL-selectin is functionally active and at high concentrations can inhibit leukocyte attachment to endothelium
• The main source for sL-selectin in serum seems to be tissue localized leukocytes
• The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors
• L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region
• Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.