CD62E (E-selectin) Monoclonal Antibody (CL-37), eBioscience™, Invitrogen™

Description

• Description: E-Selectin (CL-37) is a mouse monoclonal antibody raised against E-Selectinof human origin
• Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules
• Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells
• Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands
• This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats
• The transmembrane portion of the molecule is followed by a short cytoplasmic tail
• Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer
• Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium
• This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators
• In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation
• Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation
• Applications Tested: ELISA, Immunohistochemistry (Frozen)
• Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes
• In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation
• This response is immediate and involves a cascade of adhesion receptors
• At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade
• Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium
• E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines
• In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade
• E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.