CD31 (PECAM-1) Monoclonal Antibody (Gi18), eBioscience™, Invitrogen™

Description

• Description: The antibody BMS137 recognizes platelet endothelial cell adhesion molecule 1 (PECAM-1) also known as CD31, a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation
• PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis
• It has also been found on platelets, monocytes, neutrophils and CD8+ T cells
• Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1
• Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines
• Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction
• PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis
• Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation
• Applications Reported: This BMS137 antibody has been reported for use in ELISA, western blotting under non-reduced conditions, cell staining followed by both FACS and flow cytometric analysis, immunohistochemical staining of both frozen and FFPE tissues
• Applications Tested: This BMS137 antibody has been tested by western blot analysis and immunohistochemistry on human FFPE tissue using both low and high pH antigen retrieval at 30 μg/mL
• CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases
• CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells
• N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions
• CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis
• It is one of key regulatory molecules in vascular system.