CD31 (PECAM-1) Monoclonal Antibody (Gi18), Biotin, eBioscience™, Invitrogen™

Description

• Description: BMS137 is suitable as primary antibody in staining for FACS analysis
• BMS137FI can be used for direct staining of cells
• BMS137BT can be used in a two-step procedure using the biotin-(strept)-avidin system
• BMS137 is also suitable for Western blot analysis using non-reducing conditions
• The antibody can be used in immunohistochemistry using cryostat sections or paraffin-embedded tissue
• BMS137 is suitable for ELISA application
• PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation
• PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis
• It has also been found on platelets, monocytes, neutrophils and CD8+ T cells
• Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1
• Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines
• Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction
• PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis
• Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation
• Applications Tested: Flow Cytometry
• Purity: >95%
• CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases
• CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells
• N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions
• CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis
• It is one of key regulatory molecules in vascular system.