Deoxyribonuclease I Polyclonal Antibody, Biotin, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas]
• Cross reactivities against Deoxyribonuclease I from other sources may occur but have not been specifically determined
• Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis
• DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis
• DNase I is highly polymorphic, and at least six alleles of DNase I are known
• DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis
• DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products
• The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts
• DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers
• In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.