Mdm2Rabbit anti-Mouse, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against mouse MDM2 protein
• Reactivity occurs against Mouse MDM2 protein
• A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources
• MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53
• MDM2 is located on chromosome 12 on the q arm
• Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53
• Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53
• MDM2 has also been shown to negatively regulate p53 function
• MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function
• Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity
• Further, MDM2 plays important roles in apoptosis and cell cycle
• MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer
• In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5
• More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.